human os cell lines saos2  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Creatine Kinase Blockade Disrupts Energy Metabolism and Redox Homeostasis to Suppress Osteosarcoma Progression

doi: 10.3390/ijms262311555

Figure Lengend Snippet: Effects of CK inhibition on stemness. ( A ) Sphere-forming capacity in SaOS2 and U2OS cells following DNFB treatment. Images were taken with a phase-contrast microscope. Scale bar, 100 μm. ( B ) Expression of stemness markers Oct3 and Nestin assessed by RT-PCR. * p < 0.05 vs. C or DNFB(−). Error bars: standard deviation of three independent trials. Statistical differences were calculated using ordinary ANOVA with Bonferroni correction. ANOVA, analysis of variance; DNFB, dinitrofluorobenzene; CK, creatine kinase; OCT3, POU Class 5 homeobox 1; ACTB, β-actin.

Article Snippet: Human OS cell lines SaOS2, U2OS, HOS, MG63, and human bone marrow-derived mesenchymal stem cell line (hMSC, PCS-500-01) were purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Inhibition, Microscopy, Expressing, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

Journal: Sarcoma

Article Title: ALDH1A1 Gene Expression and Cellular Copper Levels between Low and Highly Metastatic Osteosarcoma Provide a Case for Novel Repurposing with Disulfiram and Copper

doi: 10.1155/2022/7157507

Figure Lengend Snippet: Metastatic OS cell lines and patient sarcomas display lower levels of intracellular Cu compared with nonmetastatic samples. (a) Human OS cell line triplicates (SaOS2, LM2, and LM7) were cultured under identical conditions and then collected for protein quantification and intracellular Cu analysis using atomic absorption spectrophotometry (AA). LM2 and LM7, which demonstrate increased metastatic potential, displayed significant decreases in intracellular Cu compared with parental SaOS2. (b) Deidentified patient information was used to separate sarcomas, including OS, that came from patients with metastatic disease and those with no signs of metastatic disease ( n = 14). Tumors from patients with metastatic disease displayed significantly decreased Cu levels compared with tumors from nonmetastatic patients. The statistical significance of differences among the cell types was assessed using ordinary one-way ANOVA, while the difference between the two patient tumor groups was assessed using two-tailed Student's unpaired t -test. Data represent mean ± SD: ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: The SaOS2 human OS cell line was purchased from American Type Culture Collection (ATCC).

Techniques: Cell Culture, Spectrophotometry, Two Tailed Test

Journal: Sarcoma

Article Title: ALDH1A1 Gene Expression and Cellular Copper Levels between Low and Highly Metastatic Osteosarcoma Provide a Case for Novel Repurposing with Disulfiram and Copper

doi: 10.1155/2022/7157507

Figure Lengend Snippet: Metastatic OS cell lines and patient tumors display increased ALDH expression compared with nonmetastatic samples. (a) ALDH gene expression was significantly increased in both LM2 and LM7 compared with SaOS2 cells. (b) In human patient-derived tumor cells, ALDH gene expression was significantly increased in tumor cells from patients with metastatic disease compared to patients without metastatic disease ( n = 14). The statistical significance of differences among the treatment was assessed using ordinary one-way ANOVA, while the difference between metastatic and nonmetastatic was assessed using two-tailed Student's unpaired t -test. Data represent mean ± SD: ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: The SaOS2 human OS cell line was purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Gene Expression, Derivative Assay, Two Tailed Test

Journal: Sarcoma

Article Title: ALDH1A1 Gene Expression and Cellular Copper Levels between Low and Highly Metastatic Osteosarcoma Provide a Case for Novel Repurposing with Disulfiram and Copper

doi: 10.1155/2022/7157507

Figure Lengend Snippet: Cu potentiated cytotoxicity of Dis reduces cell viability in both SaOS2 and highly metastatic LM7 cell lines. (a) SaOS2 ( n = 6) and (b) LM7 ( n = 6) cells were subjected to fold dilutions of Dox, Dis + CuCl 2 , or Dis monotherapy over 24 hours. After treatment, cells were stained with PrestoBlue viability stain, and fluorescence intensity was evaluated to measure cell viability. The addition of CuCl 2 significantly decreased the cell viability in both LM7 and SaOS2 compared to monotherapy of Dis. Dis + Cu combination treatment resulted in increased cytotoxicity to LM7 compared to Dox monotherapy.

Article Snippet: The SaOS2 human OS cell line was purchased from American Type Culture Collection (ATCC).

Techniques: Staining, Fluorescence

Journal: Sarcoma

Article Title: ALDH1A1 Gene Expression and Cellular Copper Levels between Low and Highly Metastatic Osteosarcoma Provide a Case for Novel Repurposing with Disulfiram and Copper

doi: 10.1155/2022/7157507

Figure Lengend Snippet: Combination treatment of Dox, Dis, and CuCl 2 effectively reduces viability of human OS cells and eliminates recovery in vitro . Viable cell counts of SaOS2 and LM7 were obtained using a Trypan Blue exclusion assay and a hemocytometer (colored bar graphs). After 24 h of treatment exposure, treated human OS cell lines were all recultured with fresh media without drug and monitored for recovery of cells; tan background shading indicates cells were able to grow after drug removal, and pink background shading indicates cells were unable to grow. (a) SaOS2 treated with Dox were unable to recover after a .05 µ M dose, while LM7 was unable to recover after 1.2 µ M. (b) SaOS2 and LM7 given Dis + CuCl 2 were both unable to recover after a 0.6 µ M Dis dose. (c) LM7 given triple treatment with 0.1 μ M Dis, 0.2 μ M CuCl 2 , and 0.1 μ M Dox was unable to recover after drug removal.

Article Snippet: The SaOS2 human OS cell line was purchased from American Type Culture Collection (ATCC).

Techniques: In Vitro, Trypan Blue Exclusion Assay